Research

The members of the Ovryn laboratory are interested in understanding the mechanisms that govern adhesion between the cytoskeleton and the extracellular matrix. We have developed a novel form of interference reflection microscopy to probe the dynamics of the plasma membrane in the vicinity of integrin adhesions. Additionally, we exploit several forms of fluorescent microscopy in order to probe the interplay between membrane biophysics and the myriad of proteins that assemble at an adhesion. We have developed a model, based upon statistical thermodynamic arguments, which provides a mechanistic understanding of the hierarchical processes that govern the formation of an integrin adhesion. This research is funded under grant: NIH 1R01GM076293 "Imaging Actin Dynamics at the Ventral Surface of Live Cells"

Although we seek to understand basic mechanisms, we are interested in unraveling how pathological changes are related to disease (such as cancer). Motivated by clinical observations of neonatal pulmonary disease, we are using optical microscopy to understand the mechanisms responsible for oxidant induced injury.

We are also developing optical methods to control biology. We seek to understand gene expression in single cells by applying one and two-photon uncaging microscopy methods to photoactivate a gene that subsequently begins transcription of RNA. These optical methods allow us to spatially localize gene activation and to control precisely when the activation occurs (see: http://www.nigms.nih.gov/Initiatives/CellImaging/RFA-RM-04-001.htm). This work is funded on an NIH grant: 1R01GM086217-01; Professor Robert Singer is the Principal Investigator.

Projects are available for individuals who wish to apply their quantitative backgrounds and analytical strengths to a compelling biological question. Individuals may become involved with experiments, modeling, theoretical research, image processing or the development of software.