Teaching
Professor Ovryn designs and teaches the laboratory component of the semester length course "Quantitative Imaging of Cells". During the inaugural year of the course (2007), Professor Ovryn was also a lecturer. This course, which is offered every Fall, is co-organized with Professor Erik Snapp and covers topics in modern optical and electron microscopy.
In 2009, the laboratories were made possible, in part, with generous support from the following companies:
- Carl Zeiss MicroImaging, Inc.
- Morrell Instrument Inc.
- Andor Technology
- Mad City Labs
- Invitrogen Corporation
We wish to acknowledge the following individuals for their commitment to the course:
- Nick Sillings, Carl Zeiss MicroImaging, Inc.
- Keith Gembala, Morrell Instrument Inc.
- Charles Fanghella, Andor Technology
- Dale Baldwin, Mad City Labs
- Thomas McManus, Invitrogen Corporation
In 2009, the following topics were covered in the laboratories:
I. Polarization:
- Polarization introduction laboratory
- Polarization- virtual laboratory
- Polarization- birefringence laboratory
II. Widefield microscopy laboratories:
- Anatomy of a microscope
- Köhler illumination
- Differential Interference Contrast Fluorescence
- Fluorescence
- Structured illumination
III. Detectors laboratory
IV. Total internal reflection microscopy (TIRF) laboratory
V. Home-built confocal laboratories
VI. Fluorescence recovery after photobleaching (FRAP) laboratory
VII. Fluorescence Lifetime Imaging Microscopy (FLIM) laboratories
VIII. Fluorescence resonance energy transfer (FRET) via FLIM laboratory
IX. Electron microscopy laboratory
The contribution of Matthew Nicholas to several of the laboratories is gratefully acknowledged.